Given a text file with the structure
PEPTIDE1, PEPTIDE2, PEPTIDE3
>ENSG00000000971Looking to create perl script to determine the properties of these 2 groups - the observed peptides, and the unseen peptides. Then compare the properties of the two groups (isoelectric point, length, MW, amino acid composition etc).
Looking to analyses this. To compare the "seen" peptides, with the "unseen" peptides, e..g physicochemical properties.
Wondering what properties might make certain peptides observable, others not so.
Any pointers? Or an idea through some pseudocode? I can update post and include code so far.
I can use BioPerl pepstat, emows etc I'd imagine. Or collate the physicochemical properties in an array and then export to R, but i'm happy to just get figures, then do visual analysis/ some data chewing through Perl's graph tools.